Proteomic profiling change during the early development of silicosis disease.

ABSTRACT:

Background

Silicosis is one of several severe occupational diseases for which effective diagnostic tools during early development are currently unavailable. In this study we focused on proteomic profiling during the early stages of silicosis to investigate the pathophysiology and identify the proteins involved.

Methods

Two-dimensional (2D) gel electrophoresis and MALDI-TOF-MS were used to assess the proteomic differences between healthy individuals (HI), dust-exposed workers without silicosis (DEW) and silicosis patients (SP). Proteins abundances that differed by a factor of two-fold or greater were subjected to more detailed analysis, and enzyme linked to immunosorbent assay (ELISA) was employed to correlate with protein expression data.

Results

Compared with HI, 42

SUBMITTER: Miao R 

PROVIDER: S-EPMC4805820 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature