Variable Field Analytical Ultracentrifugation: II. Gravitational Sweep Sedimentation Velocity.

ABSTRACT: Sedimentation velocity (SV) analytical ultracentrifugation is a classical biophysical technique for the determination of the size-distribution of macromolecules, macromolecular complexes, and nanoparticles. SV has traditionally been carried out at a constant rotor speed, which limits the range of sedimentation coefficients that can be detected in a single experiment. Recently we have introduced methods to implement experiments with variable rotor speeds, in combination with variable field solutions to the Lamm equation, with the application to expedite the approach to sedimentation equilibrium. Here, we describe the use of variable-field sedimentation analysis to increase the size-range covered in SV experiments by ∼100-fold with a quasi-continuous increase of rotor speed during the experi

SUBMITTER: Ma J 

PROVIDER: S-EPMC4805870 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature