Establishment of transient and stable transfection systems for Babesia ovata.

ABSTRACT:

Background

Bovine babesiosis is a tick-borne disease caused by several species of Babesia which produce acute and fatal disease in cattle and affect livestock industry worldwide. Babesia ovata is a benign species widespread in east Asian countries and causes anemia, particularly in cattle which are co-infected with Theileria orientalis. The development of genetic manipulation methods is necessary to improve our understanding of the basic biology of protozoan pathogens toward a better control of disease. Such tools have not been developed for B. ovata, and are the aim of this study.

Methods

In this study we transfected constructs that were designed to evaluate the ability of several B. ovata promoter candidates to drive expression of a reporter luciferase. We found that the e

SUBMITTER: Hakimi H 

PROVIDER: S-EPMC4806448 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature