Circulating MicroRNA-26a in Plasma and Its Potential Diagnostic Value in Gastric Cancer.

ABSTRACT:

Background

In the past decades, a good deal of studies has provided the possibility of the circulating microRNAs (miRNAs) as noninvasive biomarkers for cancer diagnosis. The aim of our study was to detect the levels of circulating miRNAs in tissues and plasmas of gastric cancer (GC) patients and evaluate their diagnostic value.

Methods

Tissue samples were collected from 85 GC patients. Plasma samples were collected from 285 GC patients and 285 matched controls. Differentially expressed miRNAs were filtered with by Agilent Human miRNA Microarray and TaqMan low density array (TLDA) with pooled samples, followed by the quantitative reverse transcription polymerase chain reaction (qRT-PCR) validation. Receiver operating characteristic (ROC) curves were structured to evaluate the

SUBMITTER: Qiu X 

PROVIDER: S-EPMC4806920 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature