ABC-F Proteins Mediate Antibiotic Resistance through Ribosomal Protection.

ABSTRACT: Members of the ABC-F subfamily of ATP-binding cassette proteins mediate resistance to a broad array of clinically important antibiotic classes that target the ribosome of Gram-positive pathogens. The mechanism by which these proteins act has been a subject of long-standing controversy, with two competing hypotheses each having gained considerable support: antibiotic efflux versus ribosomal protection. Here, we report on studies employing a combination of bacteriological and biochemical techniques to unravel the mechanism of resistance of these proteins, and provide several lines of evidence that together offer clear support to the ribosomal protection hypothesis. Of particular note, we show that addition of purified ABC-F proteins to anin vitrotranslation assay prompts dose-dependent rescu

SUBMITTER: Sharkey LK 

PROVIDER: S-EPMC4807367 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature