Specific tracking of xylan using fluorescent-tagged carbohydrate-binding module 15 as molecular probe.

ABSTRACT:

Background

Xylan has been identified as a physical barrier which limits cellulose accessibility by covering the outer surface of fibers and interfibrillar space. Therefore, tracking xylan is a prerequisite for understanding and optimizing lignocellulosic biomass processes.

Results

In this study, we developed a novel xylan tracking approach using a two-domain probe called OC15 which consists of a fusion of Cellvibrio japonicus carbohydrate-binding domain 15 with the fluorescent protein mOrange2. The new probe specifically binds to xylan with an affinity similar to that of CBM15. The sensitivity of the OC15-xylan detection approach was compared to that of standard methods such as X-ray photoelectron spectroscopy (XPS) and chemical composition analysis (NREL/TP-510-42618). All

SUBMITTER: Khatri V 

PROVIDER: S-EPMC4807533 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature