CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries.

ABSTRACT:

Background

Genetic screens using CRISPR/Cas9 are a powerful method for the functional analysis of genomes.

Results

Here we describe CRISPR library designer (CLD), an integrated bioinformatics application for the design of custom single guide RNA (sgRNA) libraries for all organisms with annotated genomes. CLD is suitable for the design of libraries using modified CRISPR enzymes and targeting non-coding regions. To demonstrate its utility, we perform a pooled screen for modulators of the TNF-related apoptosis inducing ligand (TRAIL) pathway using a custom library of 12,471 sgRNAs.

Conclusion

CLD predicts a high fraction of functional sgRNAs and is publicly available at https://github.com/boutroslab/cld.

SUBMITTER: Heigwer F 

PROVIDER: S-EPMC4807595 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature