A Simple Method for Labeling Human Embryonic Stem Cells Destined to Lose Undifferentiated Potency.

ABSTRACT: Mitochondrial oxidative phosphorylation is a major source of cellular ATP. Its usage as an energy source varies, not only according to the extracellular environment, but also during development and differentiation, as indicated by the reported changes in the flux ratio of glycolysis to oxidative phosphorylation during embryonic stem (ES) cell differentiation. The fluorescent probe JC-1 allows visualization of changes in the mitochondrial membrane potential produced by oxidative phosphorylation. Strong JC-1 signals were localized in the differentiated cells located at the edge of H9 ES colonies that expressed vimentin, an early differentiation maker. The JC-1 signals were further intensified when individual adjacent colonies were in contact with each other. Time-lapse analyses revealed that

SUBMITTER: Kumagai A 

PROVIDER: S-EPMC4807662 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature