Coordinated Changes in Gene Expression Throughout Encystation of Giardia intestinalis.

ABSTRACT: Differentiation into infectious cysts through the process of encystation is crucial for transmission and survival of the intestinal protozoan parasite Giardia intestinalis. Hitherto the majority of studies have focused on the early events, leaving late encystation poorly defined. In order to further study encystation, focusing on the later events, we developed a new encystation protocol that generates a higher yield of mature cysts compared to standard methods. Transcriptome changes during the entire differentiation from trophozoites to cysts were thereafter studied using RNA sequencing (RNA-seq). A high level of periodicity was observed for up- and down-regulated genes, both at the level of the entire transcriptome and putative regulators. This suggests the trajectory of differentiation t

SUBMITTER: Einarsson E 

PROVIDER: S-EPMC4807828 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature