Identification of cardiac-related circulating microRNA profile in human chronic heart failure.

ABSTRACT:

Background

During chronic heart failure, levels of circulating miRNAs endued with characteristics of diseased cells could be identified as biomarkers. In this study, we sought to identify cardiac-related circulating miRNAs as biomarkers of failing heart.

Methods

Total RNA of plasma and heart samples was extracted from 10 normal controls and 14 patients with chronic heart failure. Microarray was applied for miRNA profiles. Validation and organ/tissue distribution analysis was performed by qRT-PCR. In addition, bioinformatics analysis was performed to understand the critical roles of these cardiac-related circulating miRNAs in heart failure.

Results

Results showed that levels of more than half of the miRNAs dysregulated in heart failed to show any differences in plasma.

SUBMITTER: Li H 

PROVIDER: S-EPMC4807981 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature