In-depth characterization of breast cancer tumor-promoting cell transcriptome by RNA sequencing and microarrays.

ABSTRACT: Numerous studies have reported the existence of tumor-promoting cells (TPC) with self-renewal potential and a relevant role in drug resistance. However, pathways and modifications involved in the maintenance of such tumor subpopulations are still only partially understood. Sequencing-based approaches offer the opportunity for a detailed study of TPC including their transcriptome modulation. Using microarrays and RNA sequencing approaches, we compared the transcriptional profiles of parental MCF7 breast cancer cells with MCF7-derived TPC (i.e. MCFS). Data were explored using different bioinformatic approaches, and major findings were experimentally validated. The different analytical pipelines (Lifescope and Cufflinks based) yielded similar although not identical results. RNA sequencing dat

SUBMITTER: Callari M 

PROVIDER: S-EPMC4808046 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature