Light-up and FRET aptamer reporters; evaluating their applications for imaging transcription in eukaryotic cells.

ABSTRACT: The regulation of RNA transcription is central to cellular function. Changes in gene expression drive differentiation and cellular responses to events such as injury. RNA trafficking can also have a large impact on protein expression and its localization. Thus, the ability to image RNA transcription and trafficking in real time and in living cells is a worthwhile goal that has been difficult to achieve. The availability of "light-up" aptamers that cause an increase in fluorescence of their ligands when bound by the aptamer have shown promise for reporting on RNA production and localization in vivo. Here we have investigated two light-up aptamers (the malachite green aptamer and the Spinach aptamers) for their suitabilities as reporters of RNA expression in vivo using two eukaryotic cell ty

SUBMITTER: Ilgu M 

PROVIDER: S-EPMC4808412 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature