HPV status is associated with altered PIWI-interacting RNA expression pattern in head and neck cancer.

ABSTRACT:

Objectives

As HPV-induced cases of oral malignancy increase, it is important to understand the molecular differences between HPV positive and negative head and neck squamous cell carcinoma (HNSCC). PIWI-interacting RNAs (piRNAs) are a class of small non-coding RNAs aberrantly expressed in cancer. We analyzed global piRNA expression patterns to define the HNSCC piRNA transcriptome and assess whether HPV infection status associates with changes in piRNA levels.

Materials and methods

A total of 498 HNSCC small RNA sequencing libraries were acquired from the Cancer Genomics Hub (cgHUB) Data Repository and a custom sequence analysis pipeline was developed to deduce piRNA expression from raw sequencing data. Expression matrices were aligned to clinicopathological features in order

SUBMITTER: Firmino N 

PROVIDER: S-EPMC4808439 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature