Stratification of yeast cells during chronological aging by size points to the role of trehalose in cell vitality.

ABSTRACT: Cells of the budding yeast Saccharomyces cerevisiae undergo a process akin to differentiation during prolonged culture without medium replenishment. Various methods have been used to separate and determine the potential role and fate of the different cell species. We have stratified chronologically-aged yeast cultures into cells of different sizes, using centrifugal elutriation, and characterized these subpopulations physiologically. We distinguish two extreme cell types, very small (XS) and very large (L) cells. L cells display higher viability based on two separate criteria. They respire much more actively, but produce lower levels of reactive oxygen species (ROS). L cells are capable of dividing, albeit slowly, giving rise to XS cells which do not divide. L cells are more resistant to o

SUBMITTER: Svenkrtova A 

PROVIDER: S-EPMC4808460 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature