Nanohole Array-Directed Trapping of Mammalian Mitochondria Enabling Single Organelle Analysis.

ABSTRACT: We present periodic nanohole arrays fabricated in free-standing metal-coated nitride films as a platform for trapping and analyzing single organelles. When a microliter-scale droplet containing mitochondria is dispensed above the nanohole array, the combination of evaporation and capillary flow directs individual mitochondria to the nanoholes. Mammalian mitochondria arrays were rapidly formed on chip using this technique without any surface modification steps, microfluidic interconnects, or external power sources. The trapped mitochondria were depolarized on chip using an ionophore with results showing that the organelle viability and behavior were preserved during the on-chip assembly process. Fluorescence signal related to mitochondrial membrane potential was obtained from single mitocho

SUBMITTER: Kumar S 

PROVIDER: S-EPMC4809531 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature