Analysing the mutational status of adenomatous polyposis coli (APC) gene in breast cancer.

ABSTRACT:

Background

Breast cancer is a heterogeneous disorder for which the underlying genetic basis remains unclear. We developed a method for identifying adenomatous polyposis coli (APC) mutations and we evaluated the possible association between APC genetic variants and breast cancer susceptibility.

Methods

Genomic DNA was extracted from tumor and matched peripheral blood samples collected from 89 breast cancer patients and from peripheral blood samples collected from 50 controls. All samples were tested for mutations in exons 1-14 and the mutation cluster region of exon 15 by HRM analysis. All mutations were confirmed by direct DNA sequencing.

Results

We identified a new single nucleotide polymorphism (SNP), c.465A>G (K155K), in exon 4 and seven known SNPs: c.573T>C (Y191Y

SUBMITTER: Chang YS 

PROVIDER: S-EPMC4810512 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature