Functional Genomics Reveals Linkers Critical for Influenza Virus Polymerase.

ABSTRACT:

Unlabelled

Influenza virus mRNA synthesis by the RNA-dependent RNA polymerase involves binding and cleavage of capped cellular mRNA by the PB2 and PA subunits, respectively, and extension of viral mRNA by PB1. However, the mechanism for such a dynamic process is unclear. Using high-throughput mutagenesis and sequencing analysis, we have not only generated a comprehensive functional map for the microdomains of individual subunits but also have revealed the PA linker to be critical for polymerase activity. This PA linker binds to PB1 and also forms ionic interactions with the PA C-terminal channel. Nearly all mutants with five-amino-acid insertions in the linker were nonviable. Our model further suggests that the PA linker plays an important role in the conformational changes that oc

SUBMITTER: Wang L 

PROVIDER: S-EPMC4810648 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature