Identification of Novel Fusion Inhibitors of Influenza A Virus by Chemical Genetics.

ABSTRACT:

Unlabelled

A previous screening of more than 50,000 compounds led to the identification of a pool of bioactive small molecules with inhibitory effect on the influenza A virus. One of these compounds, now widely known as nucleozin, is a small molecule that targets the influenza A virus nucleoprotein. Here we identify and characterize two structurally different novel fusion inhibitors of the influenza A virus group 1 hemagglutinin (HA), FA-583 and FA-617, with low nanomolar activities. Escape mutants that are highly resistant to each of these compounds were generated, and both were found to carry mutations localized in close proximity to the B-loop of the hemagglutinin 2 protein, which plays a crucial role in the virion-host cell fusion process. Recombinant virus, generated through r

SUBMITTER: Lai KK 

PROVIDER: S-EPMC4810722 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature