ICP8 Filament Formation Is Essential for Replication Compartment Formation during Herpes Simplex Virus Infection.

ABSTRACT:

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Herpes simplex virus (HSV) dramatically reorganizes the infected-cell nucleus, leading to the formation of prereplicative sites and replication compartments. This process is driven by the essential viral single-stranded DNA (ssDNA) binding protein ICP8, which can form double-helical filaments in the absence of DNA. In this paper, we show that two conserved motifs, FNF (F1142, N1143, and F1144) and FW (F843 and W844), are essential for ICP8 self-interactions, and we propose that the FNF motif docks into the FW region during filament formation. Mammalian expression plasmids bearing mutations in these motifs (FNF and FW) were unable to complement an ICP8-null mutant for growth and replication compartment formation. Furthermore, FNF and FW mutants were able to inhibit wild-t

SUBMITTER: Darwish AS 

PROVIDER: S-EPMC4810729 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature