Mutation in mitochondrial complex I ND6 subunit is associated with defective response to hypoxia in human glioma cells.

ABSTRACT:

Background

Hypoxia-tolerant human glioma cells reduce oxygen consumption rate in response to oxygen deficit, a defense mechanism that contributes to survival under moderately hypoxic conditions. In contrast, hypoxia-sensitive cells lack this ability. As it has been previously shown that hypoxia-tolerant (M006x, M006xLo, M059K) and -sensitive (M010b) glioma cells express differences in mitochondrial function, we investigated whether mitochondrial DNA-encoded mutations are associated with differences in the initial response to oxygen deficit.

Results

The mitochondrial genome was sequenced and 23 mtDNA alterations were identified, one of which was an unreported mutation (T-C transition in base pair 14634) in the hypoxia-sensitive cell line, M010b, that resulted in a single amin

SUBMITTER: DeHaan C 

PROVIDER: S-EPMC481082 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature