Diagnostic Accuracy of Antigen 5-Based ELISAs for Human Cystic Echinococcosis.

ABSTRACT:

Background

Clinical diagnosis and follow up of cystic echinococcosis (CE) are based on imaging complemented by serology. Several immunodiagnostic tests are commercially available, but the development of new tools is still needed to overcome the lack of standardization of the target antigen, generally consisting of a crude extract of Echinococcus granulosus hydatid cyst fluid. In a previous work, we described a chromatographic method for the preparation of a highly enriched Antigen 5 fraction from hydatid cyst fluid. The high reactivity of patient sera against this preparation prompted us to evaluate further this antigen for the serodiagnosis of CE on a larger cohort of samples.

Methodology/principal findings

A total of 327 sera from CE patients with heterogeneous conditions

SUBMITTER: Pagnozzi D 

PROVIDER: S-EPMC4811537 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature