The Type III Secretion Translocation Pore Senses Host Cell Contact.

ABSTRACT: Type III secretion systems (T3SS) are nano-syringes used by a wide range of Gram-negative pathogens to promote infection by directly injecting effector proteins into targeted host cells. Translocation of effectors is triggered by host-cell contact and requires assembly of a pore in the host-cell plasma membrane, which consists of two translocator proteins. Our understanding of the translocation pore, how it is assembled in the host cell membrane and its precise role in effector translocation, is extremely limited. Here we use a genetic technique to identify protein-protein contacts between pore-forming translocator proteins, as well as the T3SS needle-tip, that are critical for translocon function. The data help establish the orientation of the translocator proteins in the host cell membra

SUBMITTER: Armentrout EI 

PROVIDER: S-EPMC4811590 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature