Vascular Cell Senescence Contributes to Blood-Brain Barrier Breakdown.

ABSTRACT:

Background and purpose

Age-related changes in the cerebrovasculature, including blood-brain barrier (BBB) disruption, are emerging as potential risks for diverse neurological conditions. Because the accumulation of senescent cells in tissues is increasingly recognized as a critical step leading to age-related organ dysfunction, we evaluated whether senescent vascular cells are associated with compromised BBB integrity.

Methods

Effects of vascular cell senescence on tight junction and barrier integrity were studied using an in vitro BBB model, composed of endothelial cells, pericytes, and astrocytes. In addition, tight junction coverage in microvessels and BBB integrity in BubR1 hypomorphic (BubR1(H/H)) mice, which display senescence cell-dependent phenotypes, were examined.<

SUBMITTER: Yamazaki Y 

PROVIDER: S-EPMC4811685 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature