Insulin-like peptide 5 is a microbially regulated peptide that promotes hepatic glucose production.

ABSTRACT:

Objective

Insulin-like peptide 5 (INSL5) is a recently identified gut hormone that is produced predominantly by L-cells in the colon, but its function is unclear. We have previously shown that colonic expression of the gene for the L-cell hormone GLP-1 is high in mice that lack a microbiota and thus have energy-deprived colonocytes. Our aim was to investigate if energy deficiency also affected colonic Insl5 expression and to identify a potential role of INSL5.

Methods

We analyzed colonic Insl5 expression in germ-free (GF), conventionally raised (CONV-R), conventionalized (CONV-D) and antibiotic-treated mice, and also assessed the effect of dietary changes on colonic Insl5 expression. In addition, we characterized the metabolic phenotype of Insl5-/- mice.

Results

We sh

SUBMITTER: Lee YS 

PROVIDER: S-EPMC4811983 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature