Discovery and dissection of metabolic oscillations in the microaerobic nitric oxide response network of Escherichia coli.

ABSTRACT: The virulence of many pathogens depends upon their ability to cope with immune-generated nitric oxide (NO·). In Escherichia coli, the major NO· detoxification systems are Hmp, an NO· dioxygenase (NOD), and NorV, an NO· reductase (NOR). It is well established that Hmp is the dominant system under aerobic conditions, whereas NorV dominates anaerobic conditions; however, the quantitative contributions of these systems under the physiologically relevant microaerobic regime remain ill defined. Here, we investigated NO· detoxification in environments ranging from 0 to 50 μM O2, and discovered a regime in which E. coli NO· defenses were severely compromised, as well as conditions that exhibited oscillations in the concentration of NO·. Using an integrated computational and experimental approach,

SUBMITTER: Robinson JL 

PROVIDER: S-EPMC4812703 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature