BMP signaling and cellular dynamics during regeneration of airway epithelium from basal progenitors.

ABSTRACT: The pseudostratified epithelium of the lung contains ciliated and secretory luminal cells and basal stem/progenitor cells. To identify signals controlling basal cell behavior we screened factors that alter their self-renewal and differentiation in a clonal organoid (tracheosphere) assay. This revealed that inhibitors of the canonical BMP signaling pathway promote proliferation but do not affect lineage choice, whereas exogenous Bmp4 inhibits proliferation and differentiation. We therefore followed changes in BMP pathway components in vivo in the mouse trachea during epithelial regeneration from basal cells after injury. The findings suggest that BMP signaling normally constrains proliferation at steady state and this brake is released transiently during repair by the upregulation of endoge

SUBMITTER: Tadokoro T 

PROVIDER: S-EPMC4813333 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature