Identification of Sleep-Modulated Pathways Involved in Neuroprotection from Stroke.

ABSTRACT:

Study objectives

Sleep deprivation (SDp) performed before stroke induces an ischemic tolerance state as observed in other forms of preconditioning. As the mechanisms underlying this effect are not well understood, we used DNA oligonucleotide microarray analysis to identify the genes and the gene-pathways underlying SDp preconditioning effects.

Design

Gene expression was analyzed 3 days after stroke in 4 experimental groups: (i) SDp performed before focal cerebral ischemia (IS) induction; (ii) SDp performed before sham surgery; (iii) IS without SDp; and (iv) sham surgery without SDp. SDp was performed by gentle handling during the last 6 h of the light period, and ischemia was induced immediately after.

Settings

Basic sleep research laboratory.

Measurements and resu

SUBMITTER: Pace M 

PROVIDER: S-EPMC4813352 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature