Loss of 5-lipoxygenase activity protects mice against paracetamol-induced liver toxicity.

ABSTRACT:

Background and purpose

Paracetamol (acetaminophen) is the most widely used over-the-counter analgesic and overdosing with paracetamol is the leading cause of hospital admission for acute liver failure. 5-Lipoxygenase (5-LO) catalyses arachidonic acid to form LTs, which lead to inflammation and oxidative stress. In this study, we examined whether deletion or pharmacological inhibition of 5-LO could protect mice against paracetamol-induced hepatic toxicity.

Experimental approach

Both genetic deletion and pharmacological inhibition of 5-LO in C57BL/6J mice were used to study the role of this enzyme in paracetamol induced liver toxicity. Serum and tissue biochemistry, H&E staining, and real-time PCR were used to assess liver toxicity.

Key results

Deletion or pharmacologic

SUBMITTER: Pu S 

PROVIDER: S-EPMC4813378 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature