Homoharringtonine binds to and increases myosin-9 in myeloid leukaemia.

ABSTRACT:

Background and purpose

Homoharringtonine (HHT) is a natural alkaloid isolated from various Cephalotaxus species. HHT has been used to treat acute myeloid leukaemia (AML), chronic myeloid leukaemia (CML), chronic lymphocyte leukaemia and myelodysplastic syndromes. Although HHT inhibits protein synthesis and promotes apoptosis of leukaemia cells in preclinical studies, its molecular target proteins remain unknown. The aim of this study was to identify target proteins of HHT.

Experimental approach

We have synthesized a biotinylated affinity column and used it to identify targets of HHT and confirmed the results by MS and Western blots. We also examined the effects of HHT on the target protein and determined roles of the target protein in anti-leukaemia activities of HHT through

SUBMITTER: Zhang T 

PROVIDER: S-EPMC4813388 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature