Immune-tolerant elastin-like polypeptides (iTEPs) and their application as CTL vaccine carriers.

ABSTRACT:

Background

Cytotoxic T lymphocyte (CTL) vaccine carriers are known to enhance the efficacy of vaccines, but a search for more effective carriers is warranted. Elastin-like polypeptides (ELPs) have been examined for many medical applications but not as CTL vaccine carriers.

Purpose

We aimed to create immune tolerant ELPs using a new polypeptide engineering practice and create CTL vaccine carriers using the ELPs.

Results

Four sets of novel ELPs, termed immune-tolerant elastin-like polypeptide (iTEP) were generated according to the principles dictating humoral immunogenicity of polypeptides and phase transition property of ELPs. The iTEPs were non-immunogenic in mice. Their phase transition feature was confirmed through a turbidity assay. An iTEP nanoparticle (NP) was as

SUBMITTER: Cho S 

PROVIDER: S-EPMC4813525 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature