Low pH and Anionic Lipid-dependent Fusion of Uukuniemi Phlebovirus to Liposomes.

ABSTRACT: Many phleboviruses (family Bunyaviridae) are emerging as medically important viruses. These viruses enter target cells by endocytosis and low pH-dependent membrane fusion in late endosomes. However, the necessary and sufficient factors for fusion have not been fully characterized. We have studied the minimal fusion requirements of a prototypic phlebovirus, Uukuniemi virus, in an in vitro virus-liposome assay. We show that efficient lipid mixing between viral and liposome membranes requires close to physiological temperatures and phospholipids with negatively charged headgroups, such as the late endosomal phospholipid bis(monoacylglycero)phosphate. We further demonstrate that bis(monoacylglycero)phosphate increases Uukuniemi virus fusion beyond the lipid mixing stage. By using electron cryo

SUBMITTER: Bitto D 

PROVIDER: S-EPMC4813561 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature