Mediator facilitates transcriptional activation and dynamic long-range contacts at the IgH locus during class switch recombination.

ABSTRACT: Immunoglobulin (Ig) class switch recombination (CSR) is initiated by the transcription-coupled recruitment of activation-induced cytidine deaminase (AID) to Ig switch regions (S regions). During CSR, the IgH locus undergoes dynamic three-dimensional structural changes in which promoters, enhancers, and S regions are brought to close proximity. Nevertheless, little is known about the underlying mechanisms. In this study, we show that Med1 and Med12, two subunits of the mediator complex implicated in transcription initiation and long-range enhancer/promoter loop formation, are dynamically recruited to the IgH locus enhancers and the acceptor regions during CSR and that their knockdown in CH12 cells results in impaired CSR. Furthermore, we show that conditional inactivation of Med1 in B cells

SUBMITTER: Thomas-Claudepierre AS 

PROVIDER: S-EPMC4813673 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature