A Study into the Collision-induced Dissociation (CID) Behavior of Cross-Linked Peptides.

ABSTRACT: Cross-linking/mass spectrometry resolves protein-protein interactions or protein folds by help of distance constraints. Cross-linkers with specific properties such as isotope-labeled or collision-induced dissociation (CID)-cleavable cross-linkers are in frequent use to simplify the identification of cross-linked peptides. Here, we analyzed the mass spectrometric behavior of 910 unique cross-linked peptides in high-resolution MS1 and MS2 from published data and validate the observation by a ninefold larger set from currently unpublished data to explore if detailed understanding of their fragmentation behavior would allow computational delivery of information that otherwise would be obtained via isotope labels or CID cleavage of cross-linkers. Isotope-labeled cross-linkers reveal cross-linke

SUBMITTER: Giese SH 

PROVIDER: S-EPMC4813691 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature