Opening a SWATH Window on Posttranslational Modifications: Automated Pursuit of Modified Peptides.

ABSTRACT: Posttranslational modifications of proteins play an important role in biology. For example, phosphorylation is a key component in signal transduction in all three domains of life, and histones can be modified in such a variety of ways that a histone code for gene regulation has been proposed. Shotgun proteomics is commonly used to identify posttranslational modifications as well as chemical modifications from sample processing. However, it favors the detection of abundant peptides over the repertoire presented, and the data analysis usually requires advance specification of modification masses and target amino acids, their number constrained by available computational resources. Recent advances in data independent acquisition mass spectrometry technologies such as SWATH-MS enable a deeper

SUBMITTER: Keller A 

PROVIDER: S-EPMC4813695 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature