Unknown

Dataset Information

0

Therapeutic Vaccination With Dendritic Cells Loaded With Autologous HIV Type 1-Infected Apoptotic Cells.


ABSTRACT: We report the results of a phase I/II, open-label, single-arm clinical trial to evaluate the safety and anti-human immunodeficiency virus type 1 (HIV-1) efficacy of an autologous dendritic cell (DC)-based HIV-1 vaccine loaded with autologous HIV-1-infected apoptotic cells.Antiretroviral therapy (ART)-naive individuals were enrolled, and viremia was suppressed by ART prior to delivery of 4 doses of DC-based vaccine. Participants underwent treatment interruption 6 weeks after the third vaccine dose. The plasma HIV-1 RNA level 12 weeks after treatment interruption was compared to the pre-ART (ie, baseline) level.The vaccine was safe and well tolerated but did not prevent viral rebound during treatment interruption. Vaccination resulted in a modest but significant decrease in plasma viremia from the baseline level (from 4.53 log10 copies/mL to 4.27 log10 copies/mL;P= .05). Four of 10 participants had a >0.70 log10 increase in the HIV-1 RNA load in plasma following vaccination, despite continuous ART. Single-molecule sequencing of HIV-1 RNA in plasma before and after vaccination revealed increases in G>A hypermutants in gag and pol after vaccination, which suggests cytolysis of infected cells.A therapeutic HIV-1 vaccine based on DCs loaded with apoptotic bodies was safe and induced T-cell activation and cytolysis, including HIV-1-infected cells, in a subset of study participants.NCT00510497.

SUBMITTER: Macatangay BJ 

PROVIDER: S-EPMC4813736 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Therapeutic Vaccination With Dendritic Cells Loaded With Autologous HIV Type 1-Infected Apoptotic Cells.

Macatangay Bernard J C BJ   Riddler Sharon A SA   Wheeler Nicole D ND   Spindler Jonathan J   Lawani Mariam M   Hong Feiyu F   Buffo Mary J MJ   Whiteside Theresa L TL   Kearney Mary F MF   Mellors John W JW   Rinaldo Charles R CR  

The Journal of infectious diseases 20151208 9


<h4>Background</h4>We report the results of a phase I/II, open-label, single-arm clinical trial to evaluate the safety and anti-human immunodeficiency virus type 1 (HIV-1) efficacy of an autologous dendritic cell (DC)-based HIV-1 vaccine loaded with autologous HIV-1-infected apoptotic cells.<h4>Methods</h4>Antiretroviral therapy (ART)-naive individuals were enrolled, and viremia was suppressed by ART prior to delivery of 4 doses of DC-based vaccine. Participants underwent treatment interruption  ...[more]

Similar Datasets

| 2226453 | ecrin-mdr-crc
2021-08-04 | E-MTAB-10414 | biostudies-arrayexpress
2021-08-04 | E-MTAB-10421 | biostudies-arrayexpress
| S-EPMC395928 | biostudies-literature