Establishment of a novel method to evaluate peritoneal microdissemination and therapeutic effect using luciferase assay.

ABSTRACT: Peritoneal dissemination is a major cause of recurrence in patients with malignant tumors in the peritoneal cavity. Effective anticancer agents and treatment protocols are necessary to improve outcomes in these patients. However, previous studies using mouse models of peritoneal dissemination have not detected any drug effect against peritoneal micrometastasis. Here we used the luciferase assay to evaluate peritoneal micrometastasis in living animals and established an accurate mouse model of early peritoneal microdissemination to evaluate tumorigenesis and drug efficacy. There was a positive correlation between luminescence intensity in in vivo luciferase assay and the extent of tumor dissemination evaluated by ex vivo luciferase assay and mesenteric weight. This model has advantages over

SUBMITTER: Takahashi R 

PROVIDER: S-EPMC4814254 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature