Proton irradiation induces persistent and tissue-specific DNA methylation changes in the left ventricle and hippocampus.

ABSTRACT:

Background

Proton irradiation poses a potential hazard to astronauts during and following a mission, with post-mitotic cells at most risk because they cannot dilute resultant epigenetic changes via cell division. Persistent epigenetic changes that result from environmental exposures include gains or losses of DNA methylation of cytosine, which can impact gene expression. In the present study, we compared the long-term epigenetic effects of whole body proton irradiation in the mouse hippocampus and left ventricle. We used an unbiased genome-wide DNA methylation study, involving ChIP-seq with antibodies to 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) to identify DNA regions in which methylation levels have changed 22 weeks after a single exposure to proton irradiation. W

SUBMITTER: Impey S 

PROVIDER: S-EPMC4815246 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature