Global histone modification profiling reveals the epigenomic dynamics during malignant transformation in a four-stage breast cancer model.

ABSTRACT:

Background

Epigenetic regulation has emerged to be the critical steps for tumorigenesis and metastasis. Multiple histone methyltransferase and demethylase have been implicated as tumor suppressors or oncogenes recently. But the key epigenomic events in cancer cell transformation still remain poorly understood.

Methods

A breast cancer transformation model was established via stably expressing three oncogenes in primary breast epithelial cells. Chromatin immunoprecipitation followed by the next-generation sequencing of histone methylations was performed to determine epigenetic events during transformation. Western blot, quantitative RT-PCR, and immunostaining were used to determine gene expression in cells and tissues.

Results

Histones H3K9me2 and me3, two repressive ma

SUBMITTER: Zhao QY 

PROVIDER: S-EPMC4815258 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature