Comparison of native and non-native ubiquitin oligomers reveals analogous structures and reactivities.

ABSTRACT: Ubiquitin (Ub) chains regulate a wide range of biological processes, and Ub chain connectivity is a critical determinant of the many regulatory roles that this post-translational modification plays in cells. To understand how distinct Ub chains orchestrate different biochemical events, we and other investigators have developed enzymatic and non-enzymatic methods to synthesize Ub chains of well-defined length and connectivity. A number of chemical approaches have been used to generate Ub oligomers connected by non-native linkages; however, few studies have examined the extent to which non-native linkages recapitulate the structural and functional properties associated with native isopeptide bonds. Here, we compare the structure and function of Ub dimers bearing native and non-native linkage

SUBMITTER: Pham GH 

PROVIDER: S-EPMC4815342 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature