Resolving the ambiguity: Making sense of intrinsic disorder when PDB structures disagree.

ABSTRACT: Missing regions in X-ray crystal structures in the Protein Data Bank (PDB) have played a foundational role in the study of intrinsically disordered protein regions (IDPRs), especially in the development of in silico predictors of intrinsic disorder. However, a missing region is only a weak indication of intrinsic disorder, and this uncertainty is compounded by the presence of ambiguous regions, where more than one structure of the same protein sequence "disagrees" in terms of the presence or absence of missing residues. The question is this: are these ambiguous regions intrinsically disordered, or are they the result of static disorder that arises from experimental conditions, ensembles of structures, or domain wobbling? A novel way of looking at ambiguous regions in terms of the pattern b

SUBMITTER: DeForte S 

PROVIDER: S-EPMC4815412 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature