E-Cadherin fragments as potential mediators for peritoneal metastasis in advanced epithelial ovarian cancer.

ABSTRACT:

Background

Peritoneal dissemination and retroperitoneal lymph node involvement are main routes for tumour spread of epithelial ovarian cancer (EOC), possibly determined by the intercellular connecting protein E-Cadherin (E-Cad) and its fragments.

Methods

Tumour tissue of 105 advanced EOC patients was evaluated for protein expression of E-Cad, β-Catenin and Calpain by western blotting and immunohistochemistry. Expression patterns were compared between tumours with solely intraperitoneal (pT3c, pN0; n=41) and tumours with retroperitoneal metastases (pT1a-3c, pN1; n=64). Lysates of the EOC cell line SKOV3 and tumour tissue from the intraperitoneal group were tested for E-Cad expression following Calpain treatment.

Results

E-Cad full-length (E-Cad-FL, 120 kDa) and two maj

SUBMITTER: Trillsch F 

PROVIDER: S-EPMC4815811 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature