AKT1-mediated Lamin A/C degradation is required for nuclear degradation and normal epidermal terminal differentiation.

ABSTRACT: Nuclear degradation is a key stage in keratinocyte terminal differentiation and the formation of the cornified envelope that comprises the majority of epidermal barrier function. Parakeratosis, the retention of nuclear material in the cornified layer of the epidermis, is a common histological observation in many skin diseases, notably in atopic dermatitis and psoriasis. Keratinocyte nuclear degradation is not well characterised, and it is unclear whether the retained nuclei contribute to the altered epidermal differentiation seen in eczema and psoriasis. Loss of AKT1 function strongly correlated with parakeratosis both in eczema samples and in organotypic culture models. Although levels of DNAses, including DNase1L2, were unchanged, proteomic analysis revealed an increase in Lamin A/C. AKT

SUBMITTER: Naeem AS 

PROVIDER: S-EPMC4816115 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature