Insights into the Structural Dynamics of Nucleocytoplasmic Transport of tRNA by Exportin-t.

ABSTRACT: Exportin-t (Xpot) transports mature 5'- and 3'-end processed tRNA from the nucleus to the cytoplasm by associating with a small G-protein Ran (RAs-related nuclear protein), in the nucleus. The release of tRNA in cytoplasm involves RanGTP hydrolysis. Despite the availability of crystal structures of nuclear and cytosolic forms of Xpot, the molecular details regarding the sequential events leading to tRNA release and subsequent conformational changes occurring in Xpot remain unknown. We have performed a combination of classical all-atom and accelerated molecular dynamics simulations on a set of complexes involving Xpot to study a range of features including conformational flexibility of free and cargo-bound Xpot and functionally critical contacts between Xpot and its cargo. The systems inves

SUBMITTER: Gupta A 

PROVIDER: S-EPMC4816717 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature