Chloroquine increases phosphorylation of AMPK and Akt in myotubes.

ABSTRACT:

Aims

There are reports that ataxia telangiectasia mutated (ATM) can activate the AMP-activated protein kinase (AMPK) and also Akt, two kinases that play integral parts in cardioprotection and metabolic function. We hypothesized that chloroquine and resveratrol, both known ATM activators, would also activate AMPK and Akt.

Main methods

Phosphorylation of AMPK and Akt was assessed after C2C12 myotubes were exposed to chloroquine or resveratrol. Additional experiments were done in cells expressing shRNA against ATM or in the presence of the ATM inhibitor KU55933. The effects of chloroquine on intracellular calcium were assessed with the fluorescent probe Calcium Green-1 AM.

Key findings

0.5 mM chloroquine increased AMPK phosphorylation by nearly 4-fold (P<0.05), and 0.25

SUBMITTER: Spears LD 

PROVIDER: S-EPMC4817090 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature