SPO11-C631T Gene Polymorphism: Association With Male Infertility and an in Silico-Analysis.

ABSTRACT:

Objective

To investigate the association of C631T single nucleotide polymorphisms in SPO11 gene with male infertilityfollowed by an in silico approach. SPO11 is a gene involved in meiosis and spermatogenesis process, which in humans, this gene is located on chromosome 20 (20q13.2-13.3) with 13 exons.

Materials and methods

In a case-control study, 200 blood samples were collected from the IVF center (Kashan, Iran) including; 100 infertile and 100 healthy control men. SPO11-C631T were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.The effects of C631T transition on the structure of mRNA and protein of SPO11 was evaluated by bioinformatics tools.

Results

Our data revealed that all subjects were wild-type homozygous in

SUBMITTER: Karimian M 

PROVIDER: S-EPMC4818377 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature