S1 domain of the porcine epidemic diarrhea virus spike protein as a vaccine antigen.

ABSTRACT:

Background

Porcine epidemic diarrhea virus (PEDV) is a highly contagious virus infecting pigs of all ages with high morbidity and mortality among newborn piglets. Currently, there is no effective vaccine available to protect the pigs from PEDV. The N-terminal subunit of spike protein (S1) is responsible for virus binding to the cellular receptor and contains a number of neutralizing antibody epitopes. Thus, we expressed and produced recombinant S1 protein to protect newborn piglets by immunization of sows.

Methods

Affinity tagged PEDV S1 protein was expressed in a secretory form in yeast, insect and mammalian cells to identify the most suitable production system. Purified recombinant protein was analysed by SDS-PAGE, Western blot and deglycosylation assay. A pregnant sow was

SUBMITTER: Makadiya N 

PROVIDER: S-EPMC4818391 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature