Kainate receptor pore-forming and auxiliary subunits regulate channel block by a novel mechanism.

ABSTRACT:

Key points

Kainate receptor heteromerization and auxiliary subunits, Neto1 and Neto2, attenuate polyamine ion-channel block by facilitating blocker permeation. Relief of polyamine block in GluK2/GluK5 heteromers results from a key proline residue that produces architectural changes in the channel pore α-helical region. Auxiliary subunits exert an additive effect to heteromerization, and thus relief of polyamine block is due to a different mechanism. Our findings have broad implications for work on polyamine block of other cation-selective ion channels.

Abstract

Channel block and permeation by cytoplasmic polyamines is a common feature of many cation-selective ion channels. Although the channel block mechanism has been studied extensively, polyamine permeation has been consid

SUBMITTER: Brown PM 

PROVIDER: S-EPMC4818602 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature