Adrenergic Stimulation of DUSP1 Impairs Chemotherapy Response in Ovarian Cancer.

ABSTRACT:

Purpose

Chronic adrenergic activation has been shown to associate with adverse clinical outcomes in cancer patients, but the underlying mechanisms are not well understood. The focus of the current study was to determine the functional and biologic effects of adrenergic pathways on response to chemotherapy in the context of ovarian cancer.

Experimental design

Increased DUSP1 production by sympathetic nervous system mediators (e.g., norepinephrine) was analyzed by real-time quantitative RT-PCR and by Western blotting. In vitro chemotherapy-induced cell apoptosis was examined by flow cytometry. For in vivo therapy, a well-characterized model of chronic stress was used.

Results

Catecholamines significantly inhibited paclitaxel- and cisplatin-induced apoptosis in ovarian c

SUBMITTER: Kang Y 

PROVIDER: S-EPMC4818718 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature