Detection of Clostridium perfringens alpha toxin gene in lambs by loop mediated isothermal amplification.

ABSTRACT:

Aim

The loop mediated isothermal amplification (LAMP) was standardized for rapid detection of Clostridium perfringens.

Materials and methods

A total of 120 fecal samples were collected from enterotoxemia suspected lambs were used for screening of C. perfringens cpa gene by LAMP. The specificity of the LAMP amplified products was tested by digesting with restriction enzyme XmnI for alpha toxin gene.

Results

Out of 120 samples screened 112 (93.3%) samples were positive by both LAMP and polymerase chain reaction (PCR) for detection of cpa gene which indicated the equal sensitivity of both the tests. The enzyme produced single cut in 162 base pair amplified product of alpha toxin gene at 81 base pair resulting in a single band in gel electrophoresis.

Conclusion

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SUBMITTER: Radhika B 

PROVIDER: S-EPMC4819352 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature