Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle.

ABSTRACT:

Aim

Out of various members of heat shock protein (HSP) superfamily which act a molecular chaperon by binding to the denaturing protein thus stabilizing them and preserving their activity, HSP70 are of major importance in thermotolerance development. Thus, present investigation aimed at a screening of HSP70 gene for polymorphisms and possible differences in thermotolerance in Tharparkar breed of cattle.

Materials and methods

A 295 bp fragment of HSP70 gene was subjected to polymerase chain reaction-single-strand conformation polymorphism (SSCP) followed by sequencing of different SSCP patterns in 64 Tharparkar cattle. A comparative thermotolerance of identified genotypes was analyzed using heat tolerance coefficients (HTCs) of animals for different seasons.

Results

Thr

SUBMITTER: Bhat S 

PROVIDER: S-EPMC4819358 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature